Luminal-Binding Protein 2 (BIP2) antibody

Antigen: Luminal-Binding Protein 2 (BIP2)

Synonyms: b-70, BIPE2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Brassica Napus, Cucumber, Rice (Oryza sativa), Daikon radish (Raphanus sativus longipinnatus), Spinach (Spinacia oleracea), Tomato (Solanum lycopersicum), Potato (Solanum tuberosum), Maize/Corn (Zea mays), Mosses, Chlamydomonas reinhardtii (C. reinhardtii)

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunolocalization (IL)

Catalog no.: ABIN349665

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP2Q39043. Chosen peptide is also conserved in Arabidopsis thaliana BiP1 protein.

Format: Lyophilized

Description: BiP2 is localized in endoplasmic reticulum lumen and plays a role in proteinassembly inside ER. Alternative name: AtBP2

Specificity: Predicted reactivity: dicots including: Nicotiana tabacum, monocots: Hordeum vulgare, Oryza sativa,trees: Picea sitchensis, Populus trichocarpa.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 600 ( IF).

Buffer: Affinity purified IgG in PBS pH 7.4

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from lyophilized material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Raphanus sativa L.Tokinashi-daikom were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane(40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fatmilk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T,each time in a fresh polystyrene box and probed with anti-BiP2 antibodies (ABIN349665,1:2000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT withagitation.

anti-Luminal-Binding Protein 2 (BIP2) antibody (Image 2)

Publications

Publications: Kobae, Uemura, Sato et al.: "Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis." in: Plant & cell physiology, Vol. 45, Issue 12, pp. 1749-58, 2005 (PubMed).

Ishikawa, Suga, Uemura et al.: "Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana." in: FEBS letters, Vol. 579, Issue 25, pp. 5814-20, 2005 (PubMed).