Catalase (CAT) antibody

Antigen: Catalase (CAT)

Synonyms: MGC138422, MGC138424, Cas1, Cs-1, Cas-1, 2210418N07, CS1, RATCATL, RATCAT01, fb68a12, wu:fb68a12, CAT, CATA, CT21282, DMCATHPO, DROCATHPO, U00145, bs36h11.y1, DmelCG6871, CG6871, MGC128112, CATALASE, CATALASE 2, T12J5.2, Cat, GB11648, catalase, MGC147040, DKFZp469E0232

Clonality: Polyclonal

Host: Rabbit

Reactivity: Ricinus communis

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349684

Additional Information

Alternative name: Catalase

Immunogen: purified native catalase from Ricinus communis

Description: Catalase is an enzyme found in most living organisms which is catalazyingdecomposition of hydrogen peroxide to water and oxygen. In plant cells catalaseis found in peroxisomes. This enzyme is involved in photorespiration andsymbiotic nitrogen fixation.

Specificity: Predicted reactivity: dicots including: Arabidopsis thaliana,Gossypium mexicanum, Vitis vinifera,monocots including: Hordeum vulgare, Oryza sativa, trees: Populus jackii.

Synonyms: MGC138422, MGC138424, Cas1, Cs-1, Cas-1, 2210418N07, CS1, RATCATL, RATCAT01, fb68a12, wu:fb68a12, CAT, CATA, CT21282, DMCATHPO, DROCATHPO, U00145, bs36h11.y1, DmelCG6871, CG6871, MGC128112, CATALASE, CATALASE 2, T12J5.2, Cat, GB11648, catalase, MGC147040, DKFZp469E0232

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Purity: ammonium sulfate purified

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from lyophilized material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

SDS-PAGE of crude preparation of catalaze purified from Ricinus communis endosperm (1)and western blot detection of crude preparation of Ricinus communis endosperm proteins (2).Proteins were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder inTBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-catalase antibodies (ABIN349684, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.