Cofilin antibody

Catalog No. ABIN7698698

The Sheep Polyclonal anti-Cofilin antibody is suitable to detect Cofilin in samples from Human. It has been validated for WB and IHC.

Quick Overview for Cofilin antibody (ABIN7698698)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Cofilin Antibody

Purpose: Sheep antibody to Cofilin-1

Specificity: Specific for Cofilin-1.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the phospho Y140 & S156 of human Cofilin-1 (Cofilin non-muscle isoform) conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat mouse and monkey.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. This antibody may also recognice the unphosphorylated form. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: Cofilin-1

Background: FUNCTION: Controls reversibly actin polymerization and depolymerization in a pH -sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. SUBCELLULAR LOCATION: Nucleus matrix. Cytoplasm cytoskeleton. Note: Almost completely in nucleus in cells exposed to heat shock or 10 % dimethyl sulfoxide. TISSUE SPECIFICITY: Widely distributed in various tissues.

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events