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Cofilin antibody (AA 2-166)

CFL1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3042747
  • Target See all Cofilin (CFL1) Antibodies
    Cofilin (CFL1) (Cofilin 1 (CFL1))
    Binding Specificity
    • 18
    • 16
    • 16
    • 15
    • 12
    • 12
    • 9
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-166
    Reactivity
    • 142
    • 95
    • 92
    • 6
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 147
    • 9
    • 2
    Rabbit
    Clonality
    • 147
    • 10
    Polyclonal
    Conjugate
    • 71
    • 12
    • 12
    • 9
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    This Cofilin antibody is un-conjugated
    Application
    • 78
    • 69
    • 47
    • 47
    • 41
    • 41
    • 31
    • 29
    • 16
    • 13
    • 7
    • 7
    • 3
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Rabbit IgG polyclonal antibody for Cofilin-1(CFL1) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Cofilin-1(CFL1) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
    Gene Name: cofilin 1 (non-muscle)
    Protein Name: Cofilin-1
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.
    Isotype
    IgG
    Top Product
    Discover our top product CFL1 Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Cofilin is approximately 0.25 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Target
    Cofilin (CFL1) (Cofilin 1 (CFL1))
    Alternative Name
    CFL1 (CFL1 Products)
    Synonyms
    cfl1 antibody, xac1 antibody, xac2 antibody, cfl antibody, cb86 antibody, sb:cb86 antibody, zgc:56501 antibody, wu:fb18a04 antibody, wu:fk77b03 antibody, 12/8 antibody, ADF antibody, ADF/cofilin antibody, Adf antibody, CG4254 antibody, Cadf antibody, D-cof antibody, D61 antibody, Dmel\\CG4254 antibody, T8 antibody, Tsr antibody, cofilin antibody, cofilin/ADF antibody, cofilin/actin depolymerizing factor homolog antibody, l(2)03101 antibody, l(2)k03101 antibody, l(2)k05633 antibody, l(2)k13409 antibody, ntf antibody, CFL antibody, AA959946 antibody, Cof antibody, CFL1 antibody, cofilin 1 (non-muscle) L homeolog antibody, cofilin 1 (non-muscle) antibody, cofilin 1 (non-muscle), like antibody, cofilin 1 antibody, twinstar antibody, cofilin antibody, cofilin 1, non-muscle antibody, Contrafreeloading antibody, cfl1.L antibody, cfl1 antibody, cfl1l antibody, CFL1 antibody, tsr antibody, COF1 antibody, TSTA_042790 antibody, Cfl1 antibody, CFL antibody
    Background
    Cofilin, a member of the ADF/cofilin family, is actually a protein with 70 % sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH , phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

    Synonyms: 18 kDa phosphoprotein antibody|CFL 1 antibody|CFL antibody|CFL1 antibody|COF1_HUMAN antibody|Cofilin 1 antibody|Cofilin 1 non muscle antibody|Cofilin antibody|Cofilin non muscle isoform antibody|Cofilin-1 antibody|non-muscle isoform antibody|p18 antibody
    Gene ID
    1072
    UniProt
    P23528
    Pathways
    Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
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