Pro-Hepcidin (AA 39-59) antibody

Catalog No. ABIN350367

This anti- antibody is a Rabbit Polyclonal antibody detecting in IHC and WB. Suitable for Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for Pro-Hepcidin (AA 39-59) antibody (ABIN350367)

Target: Pro-Hepcidin

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: AA 39-59

Purpose: Rabbit antibody to pro Hepcidin (39-59)

Specificity: Specific for Hepcidin.

Cross-Reactivity: Mouse

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 39-59 of pro region of mouse Hepcidin conjugated to blue carrier protein has been used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

References

Mohammad, Matakidou, Robbins, Lakhal-Littleton: "The kidney hepcidin/ferroportin axis controls iron reabsorption and determines the magnitude of kidney and systemic iron overload." in: Kidney international, Vol. 100, Issue 3, pp. 559-569, (2021) (PubMed).

Target Details

Target: Pro-Hepcidin

Alternative Name: pro Hepcidin

Background: FUNCTION: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. May also have antimicrobial activity. SUBCELLULAR LOCATION: Secreted. Tissue specificity: Highly expressed in the liver and to a much lesser extent in the heart.

UniProt: Q9EQ21