LIMK2 antibody (Internal Region)

Catalog No. ABIN350436

Product Details anti-LIMK2 Antibody

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Rabbit antibody to LIMK2

Specificity: Specific for LIMK2.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the internal part of human LIMK2 (LIM domain kinase 2 LIMK-2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: LIMK2 (LIMK2 Products)

Background: FUNCTION: Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. TISSUE SPECIFICITY: Highest expression in the placenta, moderate level in liver lung kidney and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver colon stomach and spleen while in brain kidney and placenta LIMK2b is the dominant form. In adult lung both LIMK2a and LIMK2b is nearly equally observed.

UniProt: P53671