GPR143 antibody (4th Cytoplasmic Domain)

Catalog No. ABIN350534

The Rabbit Polyclonal anti-GPR143 antibody has been validated for WB and IHC. It is suitable to detect GPR143 in samples from Human.

Quick Overview for GPR143 antibody (4th Cytoplasmic Domain) (ABIN350534)

Target: GPR143 (G Protein-Coupled Receptor 143 (GPR143))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR143 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GPR143 Antibody

Binding Specificity: 4th Cytoplasmic Domain

Purpose: Rabbit antibody to OA1

Specificity: Specific for OA1.

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the 4th cytoplasmic domain of human OA1 conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for GPR143

Target: GPR143 (G Protein-Coupled Receptor 143 (GPR143))

Alternative Name: OA1

Background: Ocular albinism type 1 protein is a conserved integral membrane protein with seven transmembrane domains. It is expressed in the eye and epidermal melanocytes. FUNCTION: Not known, binds heterotrimeric G proteins. SUBCELLULAR LOCATION: Melanosome membrane, Multi-pass membrane protein. Note: Targeted to intracellular organelles namely the melanosomes in pigment cells. TISSUE SPECIFICITY: Exclusively expressed in pigment cells. DISEASE: Defects in GPR143 are the cause of ocular albinism type 1 (OA1), also known as Nettleship-Falls type ocular albinism. OA1 is an X-linked disorder characterized by severe impairment of visual acuity retinal hypopigmentation and the presence of macromelanosomes.

UniProt: P51810