SNAP25 antibody

Catalog No. ABIN350909

The Sheep Polyclonal anti-SNAP25 antibody has been validated for WB and IHC. It is suitable to detect SNAP25 in samples from Mouse.

Quick Overview for SNAP25 antibody (ABIN350909)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SNAP25 Antibody

Purpose: Sheep antibody to SNAP-25

Specificity: Specific for SNAP-25.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from mouse SNAP-25 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP-25

Background: FUNCTION: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. SUBUNIT: Part of the SNARE core complex containing SNAP25 VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9 RIMS1 SNAP25BP and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1 SV2B and syntaxin-1. SUBCELLULAR LOCATION: Membrane, Lipid-anchor. Cell junction synapse synaptosome. Note: Membrane association requires palmitoylation. TISSUE SPECIFICITY: Neurons of the neocortex hippocampus piriform cortex anterior thalamic nuclei pontine nuclei and granule cells of the cerebellum.

UniProt: P60881

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport