VPS29 antibody (C-Term)

Catalog No. ABIN351326

The Rabbit Polyclonal anti-VPS29 antibody has been validated for WB and IHC. It is suitable to detect VPS29 in samples from Mouse.

Quick Overview for VPS29 antibody (C-Term) (ABIN351326)

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS29 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-VPS29 Antibody

Binding Specificity: C-Term

Purpose: Rabbit antibody to VPS29

Specificity: Specific for VPS29.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the c-terminal region of mouse VPS29 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat human chicken zebra fish and xenopus.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for VPS29

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Alternative Name: VPS29

Background: FUNCTION: Essential component of the retromer complex a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). Has no activity towards p-nitrophenylphosphate p-nitrophenylphosphorylcholine or phosphatidylinositlphosphates (in vitro). CATALYTIC ACTIVITY: O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate. COFACTOR: Binds 2 zinc ions per subunit. SUBCELLULAR LOCATION: Cytoplasm. Membrane, Peripheral membrane protein. Endosome membrane, Peripheral membrane protein.

UniProt: Q9QZ88