ALS2 antibody (AA 1644-1657)

Catalog No. ABIN351462

The Goat Polyclonal anti-ALS2 antibody (ABIN351462) specifically detects ALS2 in ELISA, IHC and IHC (p). The antibody is reactive with Human, Rat, Cow, Dog, Horse, Bat, Monkey and Rabbit samples.

Quick Overview for ALS2 antibody (AA 1644-1657) (ABIN351462)

Target: ALS2 (Amyotrophic Lateral Sclerosis 2 (Juvenile) (ALS2))

Reactivity: Human, Rat, Cow, Dog, Horse, Bat, Monkey, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This ALS2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ALS2 Antibody

Binding Specificity: AA 1644-1657

Specificity: Human ALS2.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Rat, Elephant, Dog, Bovine, Bat, Horse, Rabbit, Platypus (100%) Mouse, Xenopus (93%) Turkey, Chicken (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide LKACYYQIQREKLN from the C-terminus of human ALS2 (NP_065970.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Rat, Elephant, Dog, Bovine, Bat, Horse, Rabbit, Platypus (100%), Mouse, Xenopus (93%), Turkey, Chicken (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ALS2

Target: ALS2 (Amyotrophic Lateral Sclerosis 2 (Juvenile) (ALS2))

Alternative Name: ALS2 / Alsin

Background: Name/Gene ID: ALS2

Synonyms: ALS2, ALS2CR6, ALSJ, IAHSP, Alsin, PLSJ, KIAA1563

Gene ID: 57679

NCBI Accession: NP_065970

UniProt: Q96Q42

Pathways: Skeletal Muscle Fiber Development