ARPC1B antibody (AA 2-14)

Catalog No. ABIN351466

This Goat Polyclonal antibody specifically detects ARPC1B in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Monkey and Hamster.

Quick Overview for ARPC1B antibody (AA 2-14) (ABIN351466)

Target: ARPC1B (Actin Related Protein 2/3 Complex, Subunit 1B, 41kDa (ARPC1B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This ARPC1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARPC1B Antibody

Binding Specificity: AA 2-14

Specificity: Human ARPC1B / p41-ARC.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%) Bat, Opossum, Turkey, Chicken, Platypus, Catfish, Salmon, Sablefish, Stickleback, Pufferfish, Zebrafish (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide AYHSFLVEPISCH from the N-terminus of human ARPC1B / p41-ARC (NP_005711.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%), Bat, Opossum, Turkey, Chicken, Platypus, Catfish, Salmon, Sablefish, Stickleback, Pufferfish, Zebrafish (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ARPC1B

Target: ARPC1B (Actin Related Protein 2/3 Complex, Subunit 1B, 41kDa (ARPC1B))

Alternative Name: ARPC1B / p41-ARC / ARP2

Background: Name/Gene ID: ARPC1B

Synonyms: ARPC1B, ARC41, Arp2/3 complex 41 kd subunit, Arp2/3 complex 41 kDa subunit, p41-ARC, p40-ARC

Gene ID: 10095

NCBI Accession: NP_005711

UniProt: O15143

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization