SMARCE1 antibody (AA 364-376)

Catalog No. ABIN351474

The Goat Polyclonal anti-SMARCE1 antibody (ABIN351474) specifically detects SMARCE1 in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human, Horse, Monkey and Bat samples.

Quick Overview for SMARCE1 antibody (AA 364-376) (ABIN351474)

Target: SMARCE1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1))

Reactivity: Human, Horse, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This SMARCE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMARCE1 Antibody

Binding Specificity: AA 364-376

Specificity: Human SMARCE1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Bat, Horse (100%) Mouse, Rat, Elephant, Panda, Dog (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PPTDPIPEDEKKE from the C-terminus of human SMARCE1 (NP_003070.3). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Bat, Horse (100%), Mouse, Rat, Elephant, Panda, Dog (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000), WB (0.2 - 0.6 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SMARCE1

Target: SMARCE1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1))

Alternative Name: SMARCE1 / BAF57

Background: Name/Gene ID: SMARCE1

Synonyms: SMARCE1, BRG1-associated factor 57, BAF57

Gene ID: 6605

NCBI Accession: NP_003070

UniProt: Q969G3

Pathways: Chromatin Binding