ACSL5 antibody (AA 727-739)

Catalog No. ABIN351483

The Goat Polyclonal anti-ACSL5 antibody (ABIN351483) specifically detects ACSL5 in ELISA, WB, IHC and IHC (p). The antibody is reactive with Human and Monkey samples.

Quick Overview for ACSL5 antibody (AA 727-739) (ABIN351483)

Target: ACSL5 (Acyl-CoA Synthetase Long-Chain Family Member 5 (ACSL5))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ACSL5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACSL5 Antibody

Binding Specificity: AA 727-739

Specificity: Human ACSL5. This antibody is expected to recognise isoform a (NP_057318.2) and isoform b (NP_976313.1 and NP_976314.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Panda (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RTQIDSLYEHIQD from the C-terminus of human ACSL5 (NP_057318.2, NP_976313.1, NP_976314.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Panda (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ACSL5

Target: ACSL5 (Acyl-CoA Synthetase Long-Chain Family Member 5 (ACSL5))

Alternative Name: ACS5 / ACSL5

Background: Name/Gene ID: ACSL5
Family: Non-Protein Kinase

Synonyms: ACSL5, ACS2, ACS5, FACL5, Fatty acid coenzyme A ligase 5, Phosphatidylinositol 4-kinase, LACS 5

Gene ID: 51703

NCBI Accession: NP_057318, NP_976313, NP_976314

UniProt: Q9ULC5