RCAS1 antibody (AA 240-251)

Catalog No. ABIN351484

This Goat Polyclonal antibody specifically detects RCAS1 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rabbit, Cow, Dog, Horse, Chicken, Bat, Monkey and Pig.

Quick Overview for RCAS1 antibody (AA 240-251) (ABIN351484)

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Reactivity: Human, Mouse, Rabbit, Cow, Dog, Horse, Chicken, Bat, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This RCAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RCAS1 Antibody

Binding Specificity: AA 240-251

Specificity: Human EBAG9.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%) Rat, Lizard, Xenopus (92%) Pufferfish, Ajellomyces (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KEAQRLMKKEQN from the C-terminus of human EBAG9 (NP_004206.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%), Rat, Lizard, Xenopus (92%), Pufferfish, Ajellomyces (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RCAS1

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Alternative Name: RCAS1

Background: Name/Gene ID: EBAG9

Synonyms: EBAG9, EB9, PDAF, RCAS1

Gene ID: 9166

NCBI Accession: NP_004206

UniProt: O00559