RCAS1 antibody (AA 31-213)

Catalog No. ABIN3043569

The Rabbit Polyclonal anti-RCAS1 antibody (ABIN3043569) specifically detects RCAS1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RCAS1 antibody (AA 31-213) (ABIN3043569)

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RCAS1 Antibody

Binding Specificity: AA 31-213

Purpose: Anti-EBAG9 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-EBAG9 Antibody (ABIN3043569). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EBAG9 recombinant protein (Position: R31-S213). Human EBAG9 shares 97.8% and 94.5% amino acid (aa) sequence identity with mouse and rat EBAG9, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RCAS1

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Alternative Name: EBAG9

Background:

Synonyms: Receptor-binding cancer antigen expressed on SiSo cells,Cancer-associated surface antigen RCAS1,Estrogen receptor-binding fragment-associated gene 9 protein,EBAG9,RCAS1,

Tissue Specificity: Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.

Background: Receptor-binding cancer antigen expressed on SiSo cells is a protein that in humans is encoded by the EBAG9 gene. This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. And the encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10.

Sequence Similarities: Belongs to the MAGUK family.

Molecular Weight: 34 kDa

Gene ID: 9166

UniProt: O00559