TBC1D4 antibody (AA 825-837)

Catalog No. ABIN351486

The Goat Polyclonal anti-TBC1D4 antibody (ABIN351486) specifically detects TBC1D4 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for TBC1D4 antibody (AA 825-837) (ABIN351486)

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TBC1D4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TBC1D4 Antibody

Binding Specificity: AA 825-837

Specificity: Human TBC1D4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Orangutan, Marmoset, Rabbit (92%) Panda, Bat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DDPEKIEERKKSK from an internal region of human TBC1D4 (NP_055647.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Orangutan, Marmoset, Rabbit (92%), Panda, Bat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:128000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Alternative Name: TBC1D4 / AS160

Background: Name/Gene ID: TBC1D4

Synonyms: TBC1D4, Akt substrate of 160 kDa, AS160, KIAA0603, TBC1 domain family, member 4, TBC1 domain family member 4

Gene ID: 9882

NCBI Accession: NP_055647

UniProt: O60343