Cytokeratin 1 antibody (pan)

Catalog No. ABIN356306

The Mouse Monoclonal anti-Cytokeratin 1 antibody has been validated for IHC (p), IF and IHC (fro). It is suitable to detect Cytokeratin 1 in samples from Human.

Quick Overview for Cytokeratin 1 antibody (pan) (ABIN356306)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Cocktail

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: pan

Characteristics: Synonyms: pan Keratin, Cytokeratin Pan-reactive

Purification: Purified

Immunogen: Human epidermal keratin. Remarks: Molecular weight of antigen: CK1 (67), CK2 (65. 5), CK3 (64), CK4 (59) CK5 (58),CK6 (56), CK8 (52. 5), CK10 (56. 5), CK13 (54) CK14 (50), CK15 (50), CK16 (48), CK18 (45), CK 19(40).

Isotype: Cocktail

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for10-15 minutes followed by cooling for 10-15 minutes). Immunofluorescence. Recommended Positive Control: Human skin, lung, colon, prostate, or any GI tissue orcancer tissue's.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Cytokeratin

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).Synonyms: Cytokeratin pan-reactive, pan Keratin