HA-Tag antibody

Catalog No. ABIN356535

The Rabbit Polyclonal anti-HA-Tag antibody (ABIN356535) specifically detects HA-Tag in WB and EIA.

Quick Overview for HA-Tag antibody (ABIN356535)

Target: HA-Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-HA-Tag Antibody

Sequence: YPYDVPDYA

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/5,000. Western Blot. Immunofluorescence. (See Ref.1, 4. )ChIP. (See Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA Epitope Tag (YPYDVPDYA)

Target Type: Tag

Background: Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. This polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.