EHMT1 antibody (Center)

Catalog No. ABIN356543

The Rabbit Polyclonal anti-EHMT1 antibody has been validated for WB and EIA. It is suitable to detect EHMT1 in samples from Human.

Quick Overview for EHMT1 antibody (Center) (ABIN356543)

Target: EHMT1 (Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHMT1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-EHMT1 Antibody

Binding Specificity: Center

Specificity: This antibody is specific to EHMT1 (Center).

Purification: Protein A Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human EHMT1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for EHMT1

Target: EHMT1 (Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1))

Alternative Name: EHMT1

Background: EHMT1, also known as EUHMTASE1, is a histone methyltransferase. This protein methylates 'Lys-9' of histone H3 in vitro. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.Synonyms: EUHMTASE1, Eu-HMTase1, Euchromatic histone-lysine N-methyltransferase 1, G9a-like protein 1, GLP, GLP1, H3-K9-HMTase 5, Histone H3-K9 methyltransferase 5, Histone-lysine N-methyltransferase EHMT1, KIAA1876, KMT1D, Lysine N-methyltransferase 1D

Gene ID: 79813, 9606

UniProt: Q9H9B1