HAT1 (C-Term) antibody

Antigen: HAT1

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA)

Catalog no.: ABIN356621

Additional Information

Characteristics: Synonyms: Histone acetyltransferase type B catalytic subunit

Gene ID: 8520

Swiss-Prot: O14929

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the C-terminal region of human HAT1.

Cross-Reactivity: Human

Isotype: Ig  (Matching secondary antibodies)

Description: Histone acetylation, particularly of histone H4, has been proposed to play an importantrole in replication-dependent nucleosome assembly. The HAT1 protein contains D, A, and Bmotifs, which are present in many N-acetyltransferases, including those that acetylatesubstrates other than histones. The HAT1 holoenzyme consists of 2 subunits: the catalytic46-kD HAT1 and the accessory p46. The p46 subunit stimulated the activity of HAT1 andbound to core histones. The HAT1 holoenzyme acetylated newly synthesized but notnucleosomal histone H4 at lys5 and lys12, and, to a lesser extent, histone H2A at lys5. HAT1and p46 polypeptides are located in the nucleus of S-phase cells. HAT1 may play a role intelomeric silencing.

Specificity: This antibody is specific to HAT1 (C-term). Species: Human and Mouse. Others not tested.

Application Details

Application Notes: ELISA: 1/1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: PBS containing 0.09% (W/V) Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Restrictions: For Research Use only