SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody

Details for Product No. ABIN357469
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Antigen
Synonyms 4930431E10Rik, mKIAA1625, RGD1309707
Epitope
N-Term
(11), (11), (8), (8), (8), (6), (6), (2), (2), (2), (1), (1)
Reactivity
Human, Mouse (Murine)
(66), (25), (16), (13), (12), (12), (12)
Host
Rabbit
(50), (12), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(53), (44), (21), (14), (10), (7), (5), (5), (1), (1)
Pubmed 6 references available
Quantity 0.4 mL
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Catalog No. ABIN357469
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SMURF1.
Specificity This antibody detects SMURF1 at N-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name SMURF1
Background Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.
Alternate names: E3 ubiquitin-protein ligase SMURF1, KIAA1625, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF 1, SMURF-1
Molecular Weight 86113 Da
Gene ID 57154, 9606
UniProt Q9HCE7
Research Area Proteolysis / Ubiquitin
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Supplier Images
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody (2) anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody (Image 2)
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody (3) anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody (Image 3)
Background publications Lambris, Papamichail, Fessas: "Fluorescent labelling of proteins of lymphocyte plasma membranes." in: Journal of immunological methods, Vol. 27, Issue 1, pp. 55-9, 1979 (PubMed).

Zhu, Kavsak, Abdollah et al.: "A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation." in: Nature, Vol. 400, Issue 6745, pp. 687-93, 1999 (PubMed).

Ebisawa, Fukuchi, Murakami et al.: "Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12477-80, 2001 (PubMed).

Suzuki, Murakami, Fukuchi et al.: "Smurf1 regulates the inhibitory activity of Smad7 by targeting Smad7 to the plasma membrane." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39919-25, 2002 (PubMed).

Tajima, Goto, Yoshida et al.: "Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10716-21, 2003 (PubMed).

Kaneki, Guo, Chen et al.: "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts." in: The Journal of biological chemistry, Vol. 281, Issue 7, pp. 4326-33, 2006 (PubMed).

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