Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (C-Term) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1)

Synonyms: SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1, MGC28550, zgc:73096, wu:fc16h06, sft, ubch5, ubc4/5, ubch5a, e2(17)kb1, UBE2D1, MGC134214, MGC147208

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN357485

Additional Information

Characteristics: Synonyms: Ubiquitin-conjugating enzyme E2 D1, Ubiquitin-protein ligase D1, Ubiquitin carrier proteinD1, UBC4/5 homolog, E2(17)KB 1, UBCH5, UBCH5A

Alternative name: UBE2D1 / SFT

Swiss-Prot: P51668

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the C-terminal region of human UBE2D1.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: Ig  (Matching secondary antibodies)

Description: The modification of proteins with ubiquitin is an important cellular mechanism fortargeting abnormal or short-lived proteins for degradation. Ubiquitination involves at leastthree classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugatingenzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of irontransport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in theubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcriptionfactor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3ubiquitin-protein ligases.

Specificity: This antibody is specific to UBE2D1/SFT (C-term). Species: Human and Mouse. Others not tested. Add. Information: Predicted Molecular weight: 16602 Da

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: PBS with 0.09% (W/V) Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only