Ubiquitin-Conjugating Enzyme E2B (UBE2B) (C-Term) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2B (UBE2B)

Synonyms: HR6B, UBC2, HHR6B, RAD6B, E2-17kDa, Rad6b, mHR6B, E2-14k, 2610301N02Rik, UBE2B, fc79h10, wu:fc79h10, zgc:100921, MGC78891, hr6b, ubc2, hhr6b, rad6b, MGC89687, e2-17kda, MGC133582

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN357490

Additional Information

Characteristics: Synonyms: Ubiquitin-conjugating enzyme E2 B, Ubiquitin-protein ligase B, Ubiquitin carrier protein B,HR6B, hHR6B, E2-17 kDa

Alternative name: UBE2B / RAD6B

Swiss-Prot: P63146

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the C-terminal region of human UBE2B.

Cross-Reactivity: Human

Isotype: Ig  (Matching secondary antibodies)

Description: The modification of proteins with ubiquitin is an important cellular mechanism fortargeting abnormal or short-lived proteins for degradation. Ubiquitination involves at leastthree classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugatingenzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNAdamage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbithomologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Specificity: This antibody is specific to UBE2B/RAD6B (C-term). Predicted to cross react with Mouse and Rat (100% Antigen Homology). Species: Human. Others not tested. Add. Information: Predicted Molecular weight: 17312 Da

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: PBS with 0.09% (W/V) Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only