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Details for Product No. ABIN357529

Ubiquitin Specific Peptidase 5 (USP5) (C-Term) antibody

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Antigen
Synonyms AA407472, ISOT, ISOT-1, Ucht
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN357529
Quantity 0.4 mL
Price
346.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human USP5.
Specificity This antibody is specific to USP5/ISOT (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name USP5 / ISOT
Background Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin- related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU- domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
Alternate names: Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin carboxyl-terminal hydrolase 5, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5
Molecular Weight 95786 Da
Gene ID 8078
NCBI Accession 5874
UniProt P45974
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications Falquet, Paquet, Frutiger et al.: "cDNA cloning of a human 100 kDa de-ubiquitinating enzyme: the 100 kDa human de-ubiquitinase belongs to the ubiquitin C-terminal hydrolase family 2 (UCH2)." in: FEBS letters, Vol. 376, Issue 3, pp. 233-7, 1996 (PubMed).

Falquet, Paquet, Frutiger et al.: "A human de-ubiquitinating enzyme with both isopeptidase and peptidase activities in vitro." in: FEBS letters, Vol. 359, Issue 1, pp. 73-7, 1995 (PubMed).

Ansari-Lari, Muzny, Lu et al.: "A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13." in: Genome research, Vol. 6, Issue 4, pp. 314-26, 1996 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Alternatives for antigen "Ubiquitin Specific Peptidase 5 (USP5)", type "Antibodies"
Hosts (16), (14), (1)
Reactivities (29), (6), (4), (3), (3), (1)
Applications (25), (10), (6), (6), (3), (2), (1), (1)
Epitopes (5), (4), (3), (2), (1), (1)
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