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SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (C-Term)

Details for Product anti-SAE1 Antibody No. ABIN357744, Supplier: Login to see
Antigen
  • AOS1
  • CDGAP
  • HSPC140
  • SUA1
  • UBLE1A
  • 2400010M20Rik
  • 2610044L12Rik
  • AL033372
  • AW743391
  • D7Ertd177e
  • Sua1
  • Uble1a
  • Aos1p
  • Sua1p
  • aos
Epitope
C-Term
39
8
7
6
4
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
127
31
27
9
8
2
2
1
1
1
Host
Rabbit
83
27
16
1
Clonality
Polyclonal
Conjugate
This SAE1 antibody is un-conjugated
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
111
66
35
27
20
10
4
4
3
1
1
1
1
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AOS1.
Isotype Ig
Specificity This antibody is specific to SAE1/AOS1 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SAE1 / AOS1 (SAE1 Antibody Abstract)
Background The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A
Molecular Weight 38450 Da
Gene ID 10055, 9606
UniProt Q9UBE0
Pathways
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (C-Term) (ABIN357744) anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody
 image for anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (C-Term) (ABIN357744) anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody (Image 2)
Background publications Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).