SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody

Details for Product No. ABIN357744
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Antigen
Synonyms AOS1, CDGAP, HSPC140, SUA1, UBLE1A, 2400010M20Rik, 2610044L12Rik, AL033372, AW743391, D7Ertd177e, Sua1, Uble1a, Aos1p, Sua1p, aos
Epitope
C-Term
(20), (6), (6), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(87), (27), (25), (7), (7), (3)
Host
Rabbit
(73), (9), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(79), (54), (29), (20), (11), (10), (4), (3), (3), (2), (2)
Pubmed 3 references available
Quantity 0.4 mL
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Catalog No. ABIN357744
357.50 $
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AOS1.
Isotype Ig
Specificity This antibody is specific to SAE1/AOS1 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SAE1 / AOS1
Background The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A
Molecular Weight 38450 Da
Gene ID 10055, 9606
UniProt Q9UBE0
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody (2) anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody (Image 2)
Background publications Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Validation Images
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