anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (C-Term)

Details for Product anti-SAE1 Antibody No. ABIN357744
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Synonyms AOS1, CDGAP, HSPC140, SUA1, UBLE1A, 2400010M20Rik, 2610044L12Rik, AL033372, AW743391, D7Ertd177e, Sua1, Uble1a, Aos1p, Sua1p, aos
(38), (8), (6), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(133), (30), (28), (10), (9), (3), (1), (1), (1), (1)
(84), (32), (16), (1)
This SAE1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(120), (70), (37), (26), (22), (10), (4), (4), (4), (3), (2), (1)
Pubmed 3 references available
Quantity 0.4 mL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AOS1.
Isotype Ig
Specificity This antibody is specific to SAE1/AOS1 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SAE1 / AOS1 (SAE1 Antibody Abstract)
Background The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A
Molecular Weight 38450 Da
Gene ID 10055, 9606
UniProt Q9UBE0
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
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Background publications Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Catalog No. ABIN357744
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