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Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (pSer629) antibody

Details for Product No. ABIN358366
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Antigen
Synonyms FLH21957, HGK, MEKKK4, NIK, 9430080K19Rik, AU043147, AU045934, AW046177, Nik
Epitope
pSer629
(22), (20), (16), (12), (9), (7), (6), (6), (6), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(143), (72), (52), (49), (48), (13), (13), (1), (1)
Host
Rabbit
(124), (27)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
ELISA
(84), (60), (40), (29), (28), (21), (17), (8), (2), (1), (1)
Pubmed 3 references available
Quantity 0.4 mL
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Catalog No. ABIN358366
357.50 $
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S629 of human MAP4K4.
Specificity This antibody detects MAP4K4 pSer629.
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAP4K4
Background M4K4, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.
Alternate names: HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEKKK 4, Mitogen-activated protein kinase kinase kinase kinase 4, NIK, Nck-interacting kinase
Molecular Weight 142100 Da
Gene ID 9448
NCBI Accession 9606
UniProt O95819
Research Area Signaling
Application Notes ELISA: 1/1,000. Dot Blot: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (pSer629) antibody anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (pSer629) antibody
Background publications Ishikawa, Nagase, Suyama et al.: "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 5, Issue 3, pp. 169-76, 1998 (PubMed).

Yao, Zhou, Wang et al.: "A novel human STE20-related protein kinase, HGK, that specifically activates the c-Jun N-terminal kinase signaling pathway." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2118-25, 1999 (PubMed).

Wright, Wang, Manning et al.: "The STE20 kinase HGK is broadly expressed in human tumor cells and can modulate cellular transformation, invasion, and adhesion." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2068-82, 2003 (PubMed).

Validation Images
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