Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (pThr165) antibody

Details for Product No. ABIN358384
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Antigen
Synonyms HPK1, Hpk1, mHPK1
Epitope
pThr165
(16), (11), (10), (8), (6), (6), (6), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(105), (40), (29), (16), (16), (15)
Host
Rabbit
(90), (9), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA
(55), (53), (28), (20), (16), (15), (6)
Pubmed 3 references available
Quantity 0.4 mL
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Catalog No. ABIN358384
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T165 of human MAP4K1.
Specificity This antibody detects MAP4K1 pThr165. Predicted to cross react with Mouse (100% Antigen Homology).
Purification Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAP4K1
Background The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen- activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant- negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.
Alternate names: HPK1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEKKK 1, Mitogen-activated protein kinase kinase kinase kinase 1
Molecular Weight 91166 Da
Gene ID 11184, 9606
UniProt Q92918
Research Area Signaling
Application Notes ELISA: 1/1,000. Dot Blot: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (pThr165) antibody anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (pThr165) antibody
Background publications Hu, Qiu, Wang et al.: "Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade." in: Genes & development, Vol. 10, Issue 18, pp. 2251-64, 1996 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Wissing, Jänsch, Nimtz et al.: "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 3, pp. 537-47, 2007 (PubMed).

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