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MAP4K1 antibody (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1) (pSer171)

Details for Product anti-MAP4K1 Antibody No. ABIN358386, Supplier: Log in to see
Antigen
  • HPK1
  • Hpk1
  • mHPK1
Alternatives
anti-Human MAP4K1 antibody for ELISA
Epitope
pSer171
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19
13
13
6
6
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
124
31
25
7
7
6
1
1
1
Host
Rabbit
98
18
9
Clonality
Polyclonal
Conjugate
This MAP4K1 antibody is un-conjugated
8
8
8
7
7
7
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
70
69
35
20
16
13
9
3
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S171 of human MAP4K1.
Isotype Ig
Specificity This antibody detects MAP4K1 pSer171. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAP4K1 (MAP4K1 Antibody Abstract)
Background The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.Synonyms: HPK1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEKKK 1, Mitogen-activated protein kinase kinase kinase kinase 1
Molecular Weight 91166 Da
Gene ID 11184, 9606
UniProt Q92918
Research Area Signaling
Pathways TCR Signaling
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-MAP4K1 antibody (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1) (pSer171) (ABIN358386) anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (pSer171) antibody
Background publications Wissing, Jänsch, Nimtz et al.: "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 3, pp. 537-47, 2007 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Hu, Qiu, Wang et al.: "Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade." in: Genes & development, Vol. 10, Issue 18, pp. 2251-64, 1996 (PubMed).