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EN2 antibody (C-Term)

The Rabbit Polyclonal anti-EN2 antibody has been validated for WB and EIA. It is suitable to detect EN2 in samples from Human and Mouse.
Catalog No. ABIN359621

Quick Overview for EN2 antibody (C-Term) (ABIN359621)

Target

See all EN2 Antibodies
EN2 (Engrailed Homeobox 2 (EN2))

Reactivity

  • 60
  • 30
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Mouse

Host

  • 56
  • 3
  • 1
Rabbit

Clonality

  • 57
  • 3
Polyclonal

Conjugate

  • 25
  • 5
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This EN2 antibody is un-conjugated

Application

  • 49
  • 25
  • 13
  • 13
  • 13
  • 8
  • 6
  • 6
  • 6
  • 5
  • 3
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 18
    • 15
    • 8
    • 6
    • 1
    C-Term

    Specificity

    This antibody reacts to EN2.

    Purification

    Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human EN2.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    EN2 (Engrailed Homeobox 2 (EN2))

    Alternative Name

    Engrailed-2 / EN2

    Background

    EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.Synonyms: EN-2, Homeobox protein engrailed-2, Hu-En-2

    Gene ID

    2020, 9606

    UniProt

    P19622
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