14-3-3 zeta antibody (Thr232)

Catalog No. ABIN360667

This Rabbit Polyclonal antibody specifically detects 14-3-3 zeta in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for 14-3-3 zeta antibody (Thr232) (ABIN360667)

Target: 14-3-3 zeta (YWHAZ)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-14-3-3 zeta Antibody

Binding Specificity: Thr232

Specificity: This antibody reacts to 14-3-3 protein zeta/delta.

Purification: Protein A column, followed by peptide affinity purification

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T232 of human 14-3-3.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: 14-3-3 Protein zeta/delta

Background: 14-3-3 protein zeta/delta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ

Gene ID: 7534, 9606

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location