NBL1 antibody

Catalog No. ABIN360878

The Rat Monoclonal anti-NBL1 antibody is suitable to detect NBL1 in samples from Mouse. It has been validated for WB.

Quick Overview for NBL1 antibody (ABIN360878)

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This NBL1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 7K12

Product Details anti-NBL1 Antibody

Specificity: This antibody detects Mouse DAN in Western blotting.

Purification: Protein A/G affinity chromatography

Immunogen: Recombinant DAN

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.2 mL sterile PBS and the final concentration is 0.5 mg/mL.

Buffer: 0.2 μm filtered PBS, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NBL1

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Alternative Name: NBL1

Background: DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in srctransformed rat fibroblasts. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative BMP antagonists. DAN family members share a cysteinerich domain that is structurally related to the cysteineknot motif found in TGFβ superfamily ligands. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberusrelated), Dante and PRDC (protein-related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chick Caronte, and C. elegans CeCan 1. Murine DAN binds BMP2 in immunoprecipitation experiments and acts as a BMP antagonist in Xenopus animal cap explants. The DAN family of proteins are thought to act as antagonists by binding BMP ligands and preventing their interactions with signaling receptor complexes. Recombinant mouse DAN have been shown to bind BMP4 in a functional ELISA. It is likely the various DAN family members and other TGFβ BMP antagonists including Noggin, Chordin, Follistatin, and TSG can selectively antagonize the activities of different subsets of TGFβ superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGFβ superfamily ligands.Synonyms: DAN, DAND1, Neuroblastoma suppressor of tumorigenicity 1

Gene ID: 17965

NCBI Accession: NP_032701

UniProt: Q61477