VEGF 165b (C-Term) antibody

Catalog No. ABIN360957

The Mouse Monoclonal anti-VEGF 165b antibody (Clone 7F17) (ABIN360957) specifically detects VEGF 165b in WB, IHC (fro) and IHC (p). The antibody is reactive with Human samples.

Quick Overview for VEGF 165b (C-Term) antibody (ABIN360957)

Target: VEGF 165b

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7F17

Product Details

Binding Specificity: C-Term

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Sterility: 0.2 μm filtered

Immunogen: Recombinant Human VEGF-165b C-terminal peptide

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1/100 - 1/1000. Immunohistochemistry on frozen section: 1/20 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL sterile PBS to the concentration of 0.2 mg/mL

Concentration: 0.2 mg/mL

Buffer: Lyophilized from a 0.2 μm filtered solution in Phosphate-Buffered Saline (PBS)

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: VEGF 165b

Background: VEGF165b is an mRNA splice variant of VEGF. Alternative exon usage results in a 165 aa product that is identical to VEGF165 except for 6 unique amino acids at the C-terminus. VEGF165b inhibits proliferation and migration of endothelial cells and is down-regulated in renal cell carcinomas.Synonyms: VEGF, VEGF-A, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor