Distal-Less Homeobox 5 (DLX5) antibody

Antigen: Distal-Less Homeobox 5 (DLX5)

Synonyms: AI385752, RDLX, dll3, MGC69418, DLX5

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN361189

Additional Information

Alternative name: DLX5

Gene ID: 1749, 13395 (mouse)

Immunogen: Peptide with sequence C-AYNRVPSATNQPEK, from the internal region of the protein sequence according to NP_005212.1

Description: All names and symbols: DLX5, distal-less homeobox 5, distal-less homeo box 5. Official Symbol: DLX5. RefSeq number(s): NP_005212.1.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Cow, Internal Region

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 30kDa band observed in Human Bone Marrow lysates (calculated MW of 31.5kDa according to NP_005212.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, J Biol Chem. 2003 Sep 5,278(36):34387-94, PMID: 12815054). Recommended concentration: 1-3µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

EB09284 (1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.