ATP Synthase Subunit delta (AtpH) antibody

Antigen: ATP Synthase Subunit delta (AtpH)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii (C. reinhardtii)

Application: Western Blotting (WB)

Catalog no.: ABIN361271

Additional Information

Alternative name: ATP synthase subunit c (AtpH)

Immunogen: KLH-conjugated peptides derived fromAtpH subunit c of Arabidopsis thalianaP56760 and Chlamydomonas reinhardtii Q37304

Format: Lyophilized

Description: F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATPfrom ADP and phosphate using the energy stored in a transmembrane iongradient. Multiple copies of the c subunit build up the ring structure (in spinach a14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

Specificity: Predicted reactivity: dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing:Hordeum vulgare, Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii,moss: Physcomitrella patens, green algae, Ostreococcus tauri.

Application Details

Application Notes: Recommended Dilution 1: 10 000 with standard ECL (WB).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1),Chlamydomonas reinhardtii subunit gamma deletion mutant thylakoid membrane fraction(2), Arabidospsis thaliana thylakoid membrane fraction (3), Chlamydomonas reinhardtiithylakoid membrane preparation (4) were separated on 12-18% acrylamide-8M urea geland blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 xPBS and probed with anti-ATP synthase subunit c antibody (AS09 591, 1: 10 000, 1h) andsecondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5%dry milk. All steps were performed at RT with agitation. Signal was detected with standardECL (GE Healthcare), exposure time 30’’. Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) Anovel pathway of cytochrome c biogenesis is involved in the assembly of the cytochromeb6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 andChlamydomonas membranes were prepared according to Chua & Bennoun (1975)Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutantstrains deficient in photosystem II reaction center. PNAS 72:2175-2179 Courtesy Dr. Yves Choquet 1