AtpH antibody

Catalog No. ABIN249344

The Rabbit Polyclonal anti-AtpH antibody is suitable to detect AtpH in samples from Arabidopsis thaliana, Nicotiana benthamiana, Spinach and Thermosynechococcus elongatus. It has been validated for WB.

Quick Overview for AtpH antibody (ABIN249344)

Target: AtpH

Reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Spinach, Thermosynechococcus elongatus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-AtpH | ATP synthase subunit c (chloroplastic)

Specificity: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases.

Cross-Reactivity (Details): Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Algae,Cannabis sativa,Cyclotella cryptica,Glycine max,Oryza sativa,Physcomitrium patens,Phaeodactylum tricornutum,Pisum sativum,Populus alba,Pinus thunbergii,Thalassiosira pseudonana,Zea mays,Vitis vinifera

Purification: Serum

Immunogen: Purified c subunit from Spinacia oleracea UniProt: P69447.

Application Details

Application Notes: 1 : 1000-1 : 10 000 (WB)

Comment:

please note that increased incubation at 95°C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: AtpH

Background:

Background: F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

Additional information: This product can be sold containing proclin if requested.

Molecular Weight: 8 kDa (for Arabidopsis thaliana)

Gene ID: 2715579

UniProt: P69447