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Actin antibody
| Antigen | Actin |
| Synonyms |
A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G ...
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| Clonality | Monoclonal (C4) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives All Species |
| Application |
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
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| Catalog no. | ABIN361349 |
| Quantity | 100 µl |
| Price | 295.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 to 7 Business days |
Additional Information
| Isotype | IgG1 (Matching secondary antibodies) |
| Clone | C4 |
| Description | Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Anti-Actin Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein. |
| Specificity | Specific for the ~42 kDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms. |
| Synonyms | A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051 |
Application Details
| Application Notes | Recommended Dilution: WB:1:5,000 IF: 1:500 IHC: 1:500 Quality Control: Western blots performed on each lot. |
| Purification | Unpurified ascites fluid. |
| Buffer | Antibody + 10mM NaN3 |
| Storage | Store at -20 °C, stable for 1 year |
| Research Area | Cancer, Apoptosis/Necrosis, Cytoskeleton, Microfilaments, Extracellular Matrix |
| Restrictions | For Research Use only |
Images
| Western blots of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein. |
Alternatives
Alternatives for antigen "Actin", type "Antibodies"
| Hosts | Mouse (58), Rabbit (18), Sheep (3) |
| Reactivities | Human (47), Chicken (23), Rat (Rattus) (20), Mouse (Murine) (12), Cow (Bovine) (11), Rabbit (10), Snake (8), Carp (6), Frog (6), Guinea Pig (6), Sheep (Ovine) (5), Dog (Canine) (3), All Species (2), Cat (Feline) (2), Pig (Porcine) (2), Xenopus laevis (2), Dictyostelium (1), Horse (Equine) (1), Physarum (1), Primate (1), Primates (1) |
| Applications | Western Blotting (WB) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (38), Immunofluorescence (IF) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), ELISA (16), Immunohistochemistry (IHC) (15), Immunoprecipitation (IP) (14), Immunocytochemistry (ICC) (7), Enzyme Immunoassay (EIA) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (EM) (1), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (6), Cy3 (1), FITC (1) |
| Epitopes | C-Term (6), pan (6), N-Term (3), Ab-55/53 (1), Asp244 (1), pTyr55/53 (1) |
