Actin antibody (AA 300-380)

Catalog No. ABIN7200793

The Rabbit Polyclonal anti-Actin antibody (ABIN7200793) specifically detects Actin in WB, IHC, IF and ELISA. The antibody is reactive with Human, Rat, Mouse and Fish samples.

Quick Overview for Actin antibody (AA 300-380) (ABIN7200793)

Target: Actin

Reactivity: Human, Rat, Mouse, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-Actin Antibody

Binding Specificity: AA 300-380

Purpose: Rabbit Anti-Actin Polyclonal Antibody

Specificity: Actin Polyclonal Antibody detects endogenous levels of Actin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Actin at AA range: 300-380

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Actin

Target: Actin

Background: ACTB, Actin, cytoplasmic 1, Beta-actin, POTEKP, ACTBL3, FKSG30, Putative beta-actin-like protein 3, Kappa-actin, POTE ankyrin domain family member K, ACTG1, ACTB, ACTG, Actin, cytoplasmic 2, Gamma-actinACTB encodes one of six different actin proteins (actin beta). Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Molecular Weight: 45kD

Gene ID: 60, 71

UniProt: P60709, Q9BYX7, P63261