Periostin, Osteoblast Specific Factor (POSTN) (pan) antibody

Antigen: Periostin, Osteoblast Specific Factor (POSTN)

Synonyms: PN, OSF2, OSF-2, PDLPOSTN, MGC119510, MGC119511, periostin, RP11-412K4.1, PLF, Osf2, peri, AI747096, Periostin, MGC128214, pn, fa99h07, MGC153873, wu:fa99h07, wu:fc70f09, zgc:153873, LOC100008945, osf-2, pdlpostn

Epitope: pan

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Chicken, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361362

Additional Information

Alternative name: Periostin, pan

Immunogen: peptide from fasciclin domain 1 of mouse periostin.

Description: Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008).

Specificity: The antibody has been directly tested for reactivity in chicken, human, mouse and rat. Based on the homology of the immunogen with other species, we expect that this antibody will recognize periostin in most, if not all, species of mammals, birds, amphibians, and fishes. Specific for three periostin bands around ~93 kDa in mouse lung extract. The immunogenic peptide is present in all known splice variants of periostin and therefore this antibody recognizes all known molecular forms of periostin. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigenretrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20).

Synonyms: PN, OSF2, OSF-2, PDLPOSTN, MGC119510, MGC119511, periostin, RP11-412K4.1, PLF, Osf2, peri, AI747096, Periostin, MGC128214, pn, fa99h07, MGC153873, wu:fa99h07, wu:fc70f09, zgc:153873, LOC100008945, osf-2, pdlpostn

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IHC: 1:100 Quality Control: Western blots performed on each lot.

Concentration: ~ 0.5 mg/ml

Purification: Affinity purified rabbit serum.

Buffer: Liquid in PBS. Contains no preservative.

Storage: Store at -20°C in undiluted aliquots; stable for up to six months after date of receipt. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western blots of rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein triplet.