Collagen, Type I, 1 Propeptide Sequence antibody

Antigen: Collagen, Type I, 1 Propeptide Sequence

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361374

Additional Information

Alternative name: Collagen I (1 Propeptide Sequence

Description: Collagen is the most abundant protein in mammals with type 1 collagen being the most abundant of the 29 known collagens. Type 1 collagen is integral in the strengthening and structural support of many tissues including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procoallagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005). Anti-Collagen I 1, propeptide

Specificity: Specific for the ~180 kDa collagen I (1 propeptide in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, it recognizes collagen I molecules that are still associated with the cells in which they were synthesized.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:100 Quality Control: Western blots performed on each lot.

Purification: Affinity purified rabbit serum.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blot of rat lung lysate showing specific immunolabeling of the ~180 k collagen 1