gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2) (pSer327) antibody

Antigen: gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2)

Synonyms: gamma2, GABAA-R, Gabrg-2, AI851231, BB128510, GABRG2, gabrg1, DKFZp459N1126, CAE2, ECA2, GEFSP3

Epitope: pSer327

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361470

Additional Information

Alternative name: Phospho-Ser327 GABAA Receptor, gamma2 subunit

Format: Affinity-purified

Description: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha and beta subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma subunit is required for benzodiazepine modulation. It has recently been suggested that PKC regulates the sensitivity of GABAA alpha1beta2gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2 (Qi et al., 2007)

Specificity: Specific for ~45k GABAA receptor gamma2 subunit phosphorylated at Ser327. Immunolabeling of the GABAA band is completely blocked by lambda phosphatase treatment.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat cortex showing specific immunolabeling of the ~45k GABAA gamma2 protein phosphorylated at Ser327 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).