gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1) (pSer923) antibody

Antigen: gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1)

Synonyms: GB1, GPRC3A, GABABR1, FLJ92613, GABBR1-3, dJ271M21.1.1, dJ271M21.1.2, GABBR1, MGC140191, GABA(B)R1, DKFZp459L2125

Epitope: pSer923

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361473

Additional Information

Alternative name: Phospho-Ser923 GABAB R1

Format: Affinity-purified

Description: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. Phosphorylation enhances GABAB receptor effector coupling (Couve et al., 2004). Phosphorylation of Ser923 is thought to be important in the regulation of GABAB receptor function. Anti-Phospho-Ser923 GABAB R1 Western blot of rat synaptic membrane showing specific immunolabeling of the ~102k GABAB R1 protein phosphorylated at Ser923 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser923 GABAB antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Specificity: Specific for ~102k GABAB R1 phosphorylated at Ser923. Immunolabeling of the GABAB R1 band is completely blocked by (-phosphatase treatment.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat synaptic membrane showing specific immunolabeling of the ~102k GABAB R1 protein phosphorylated at Ser923 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser923 GABAB antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.