Cdc2 (CDK1) (pTyr15) antibody

Antigen: Cdc2 (CDK1)

Epitope: pTyr15

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Xenopus laevis

Application: Western Blotting (WB)

Catalog no.: ABIN361481

Additional Information

Alternative name: Phospho-Tyr15 cdc2

Format: Affinity-purified

Description: Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003, Dranovsky et al., 2001, Okano et al., 1996). Anti-Phospho Tyr15 cdc2 Western blot of human T47D cells showing specific immunolabeling of the ~34k cdc2 phosphorylated at Tyr15 (Control). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: (-Ptase). These blots are identical to the control except that they were incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr15 cdc2. The immunolabeling is completely eliminated by treatment with (-Ptase.

Specificity: Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the (-phosphatase treatment.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of human T47D cells showing specific immunolabeling of the ~34k cdc2 phosphorylated at Tyr15 (Control). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: (-Ptase). These blots are identical to the control except that they were incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr15 cdc2. The immunolabeling is completely eliminated by treatment with (-Ptase.