MEK1/2 antibody (pSer218, pSer222)

Catalog No. ABIN361507

This Rabbit Polyclonal antibody specifically detects MEK1/2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for MEK1/2 antibody (pSer218, pSer222) (ABIN361507)

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1/2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MEK1/2 Antibody

Binding Specificity: pSer218, pSer222

Specificity: Specific for endogenous levels of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218/222. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Human, Mouse

Predicted Reactivity: bovine, canine, chicken, non-human primate, rat, Xenopus, zebra fish

Characteristics: Our MEK1/2 (Ser218,222) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects MEK1/2 (Ser218,222) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 of human MEK1/2, conjugated to keyhole limpet hemocyanin (KLH). Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222, the phosphosites in mouse MEK 1/2 are Ser217 and Ser221.

Isotype: IgG

Application Details

Application Notes: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

Target Details for MEK1/2

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Alternative Name: MAP2K1/2

Background:

Synonyms: AA589381 antibody, CFC3 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 2 antibody, EC 2.7.12.2 antibody, ERK activator kinase 1 antibody, ERK activator kinase 2 antibody, FLJ26075 antibody, MAP kinase kinase 1 antibody, MAP kinase kinase 2 antibody, MAP2K1 antibody, MAP2K2 antibody, MAPK/ERK kinase 1 antibody, MAPK/ERK kinase 2 antibody, MAPKK 1 antibody, MAPKK1 antibody, MAPKK2 antibody, MEK 1 antibody, MEK1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, Mitogen activated protein kinase kinase 2 antibody, Mitogen-activated protein kinase kinase 2, p45 antibody, MK2 antibody, MKK 1 antibody, MKK 2 antibody, MKK1 antibody, MKK2 antibody, MP2K1_HUMAN antibody, PRKMK 1 antibody, PRKMK 2 antibody, Prkmk1 antibody, Prkmk2 antibody, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody, Protein kinase, mitogen-activated, kinase 1 antibody, Protein kinase, mitogen-activated, kinase 2 antibody

Description Logic: Our MEK1/2 (Ser218,222) rabbit polyclonal phosphospecific primary antibody is produced . It detects MEK1/2 (Ser218,222) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: MAP2K1/2

Molecular Weight: 45 kDa

Gene ID: 5604

UniProt: Q02750