Calcium/calmodulin-Dependent Protein Kinase II (CAMK2) antibody

Antigen: Calcium/calmodulin-Dependent Protein Kinase II (CAMK2)

Synonyms: CAMKA, KIAA0968, CaMKII, R74975, mKIAA0968, PKCCD, PK2CDD, CAMKII, CAMKIIalpha, CAMKIId, CDPK1, Ca2+/calmodulin-dependent protein kinase II, CaM, CaM II, CaM KII, CaMK II, CaMK-II, Calcium/calmodulin-dependent protein kinase, DCK, calcium/calmodulin-dependent protein kinase II, dCaMKII, DmelCG18069, CG18069, CAMK2A, MGC139375, MGC155201, MGC123320, zgc:112538, zgc:123320, Camkii, GB17817, DKFZp459L1326

Clonality: Monoclonal (6G9)

Host: Mouse

Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361643

Additional Information

Alternative name: CaMKII

Immunogen: Partially purified rat CaMKII

Format: Protein G purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 6G9

Description: CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Specificity: Detects ~50-60kDa protein, corresponding to the apparent molecular mass of CaMKII a subunit on SDS-PAGE immunblots. Both the phosphorylated and non-phosphorylated forms are recognized. Species cross-reactivity: Mouse, Rat, Bovine, Human.

Synonyms: CAMKA, KIAA0968, CaMKII, R74975, mKIAA0968, PKCCD, PK2CDD, CAMKII, CAMKIIalpha, CAMKIId, CDPK1, Ca2+/calmodulin-dependent protein kinase II, CaM, CaM II, CaM KII, CaMK II, CaMK-II, Calcium/calmodulin-dependent protein kinase, DCK, calcium/calmodulin-dependent protein kinase II, dCaMKII, DmelCG18069, CG18069, CAMK2A, MGC139375, MGC155201, MGC123320, zgc:112538, zgc:123320, Camkii, GB17817, DKFZp459L1326

Application Details

Application Notes: 0.1ug/mL of SMC124 was needed for detection of CaMKII in 20 ug of rat brain tissue

Concentration: 1.0mg/mL

Buffer: Mouse immunoglobulin in PBS pH 7.4, 0.09% sodium azide, in 50% glycerol. Protein G purified.

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: Cancer, Hypertrophy, Kinases/Phosphatases

Restrictions: For Research Use only

Images

CamKII (6G9) in cultured cortical neurons (E18P21)

Publications

Publications: Bennett, Kennedy: "Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 7, pp. 1794-8, 1987 (PubMed).

Brocke, Srinivasan, Schulman: "Developmental and regional expression of multifunctional Ca2+/calmodulin-dependent protein kinase isoforms in rat brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 10, pp. 6797-808, 1995 (PubMed).

Edman, Schulman: "Identification and characterization of delta B-CaM kinase and delta C-CaM kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms." in: Biochimica et biophysica acta, Vol. 1221, Issue 1, pp. 89-101, 1994 (PubMed).

Nghiem, Saati, Martens et al.: "Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues." in: The Journal of biological chemistry, Vol. 268, Issue 8, pp. 5471-9, 1993 (PubMed).

Tombes, Krystal: "Identification of novel human tumor cell-specific CaMK-II variants." in: Biochimica et biophysica acta, Vol. 1355, Issue 3, pp. 281-92, 1997 (PubMed).

Barria, Muller, Derkach et al.: "Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5321, pp. 2042-5, 1997 (PubMed).

Strack, Colbran: "Autophosphorylation-dependent targeting of calcium/ calmodulin-dependent protein kinase II by the NR2B subunit of the N-methyl- D-aspartate receptor." in: The Journal of biological chemistry, Vol. 273, Issue 33, pp. 20689-92, 1998 (PubMed).

Leonard, Lim, Hemsworth et al.: "Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 3239-44, 1999 (PubMed).

Makhinson, Chotiner, Watson et al.: "Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 7, pp. 2500-10, 1999 (PubMed).

Shen, Meyer: "Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 162-6, 1999 (PubMed).

Means: "Regulatory cascades involving calmodulin-dependent protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 1, pp. 4-13, 2000 (PubMed).

Hughes, Edin, Antonsson et al.: "Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36008-13, 2001 (PubMed).