Calcium/calmodulin-Dependent Protein Kinase II (CAMK2) antibody

Antigen: Calcium/calmodulin-Dependent Protein Kinase II (CAMK2)

Synonyms: CAMKA, KIAA0968, CaMKII, R74975, mKIAA0968, PKCCD, PK2CDD, CAMKII, CAMKIIalpha, CAMKIId, CDPK1, Ca2+/calmodulin-dependent protein kinase II, CaM, CaM II, CaM KII, CaMK II, CaMK-II, Calcium/calmodulin-dependent protein kinase, DCK, calcium/calmodulin-dependent protein kinase II, dCaMKII, DmelCG18069, CG18069, CAMK2A, MGC139375, MGC155201, MGC123320, zgc:112538, zgc:123320, Camkii, GB17817, DKFZp459L1326

Clonality: Monoclonal (6G9)

Host: Mouse

Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA

Catalog no.: ABIN361644

Additional Information

Characteristics: Detects ~50-60kDa protein, corresponding to the molecular mass of CaMKII alpha-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms.

Alternative name: CaMKII

Gene ID: 12322

Swiss-Prot: P11798

Immunogen: Partially purified rat CaMKII

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 6G9

Description: CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+ auto inhibitory effect and activates the kinase. /calmodulin to its regulatory domain releases its This kinase activation results in autophosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Synonyms: CamK2, CamK2A, CamK2B, CamK2D, CamK2G, CAMKA

Specificity: Detects ~50-60kDa protein, corresponding to the apparent molecular mass of CaMKII a subunit on SDS-PAGE immunblots. Both the phosphorylated and non-phosphorylated forms are recognized. Species cross-reactivity: Mouse, Rat, Bovine, Human.

Sensitivity: 0.1µg/ml was sufficient for detection of CamKII in 20µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Synonyms: CAMKA, KIAA0968, CaMKII, R74975, mKIAA0968, PKCCD, PK2CDD, CAMKII, CAMKIIalpha, CAMKIId, CDPK1, Ca2+/calmodulin-dependent protein kinase II, CaM, CaM II, CaM KII, CaMK II, CaMK-II, Calcium/calmodulin-dependent protein kinase, DCK, calcium/calmodulin-dependent protein kinase II, dCaMKII, DmelCG18069, CG18069, CAMK2A, MGC139375, MGC155201, MGC123320, zgc:112538, zgc:123320, Camkii, GB17817, DKFZp459L1326

Application Details

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Cancer, Hypertrophy, Kinases/Phosphatases

Restrictions: For Research Use only

Images

CamKII (6G9) in cultured cortical neurons (E18P21)