KDEL Receptor (KDELR) antibody

Antigen: KDEL Receptor (KDELR)

Synonyms: BEST:CK00230, CK00230, ERD2, dmErd2, l(2)k00311, DmelCG5183, CG5183

Clonality: Monoclonal (KR-10)

Host: Mouse

Reactivity: Human, Monkey, Rat (Rattus), Mouse (Murine), Hamster, Rabbit, Pig (Porcine), Cow (Bovine), Sheep (Ovine), Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster), Xenopus laevis

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Catalog no.: ABIN361676

Additional Information

Characteristics: Detects an ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots.

Alternative name: KDEL Receptor

Gene ID: 68137

Swiss-Prot: Q99JH8

Immunogen: A 21 residue synthetic peptide based on the bovine KDEL receptor and the peptide coupled to KLH

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: KR-10

Description: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention. There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity, and all are located within the cis-Golgi and its intermediate compartments. In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding. It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal.
Synonyms: ERD2, ERD2.1, ERD21, HDEL, KDEL, KDEL R1, KDELR1, PM23

Specificity: Detects an ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots. Species cross-reactivity: Human, Monkey, Mouse, Rat, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosphilia, Xenopus.

Sensitivity: 1µg/ml was sufficient for detection of KDEL receptor in 20µg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Application Details

Concentration: 1 mg/mL

Purification: Mouse Ascites fluid

Buffer: PBS pH7.2, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Receptors

Restrictions: For Research Use only

Images

KDEL Receptor (KR-10), merged pic of KR-10 (red) and DAPI (blue) in NRK cells. Courtesy of Institute of Mol. and Cell Bio, Singapore