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KDELR antibody (AA 192-212) (PE)

KDELR Reactivity: Cow WB, IP, IHC, ICC, IF Host: Mouse Monoclonal KR-10 PE
Catalog No. ABIN2481611
  • Target See all KDELR Antibodies
    KDELR (KDEL Receptor (KDELR))
    Binding Specificity
    • 19
    • 1
    AA 192-212
    Reactivity
    • 22
    • 13
    • 13
    • 12
    • 12
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 2
    • 1
    Cow
    Host
    • 22
    • 1
    Mouse
    Clonality
    • 22
    • 1
    Monoclonal
    Conjugate
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This KDELR antibody is conjugated to PE
    Application
    • 22
    • 21
    • 21
    • 11
    • 10
    • 9
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Detects ~25 kDa.
    Cross-Reactivity
    Chicken, Cow, Dog, Drosophila melanogaster, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis
    Purification
    Protein G Purified
    Immunogen
    A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
    Clone
    KR-10
    Isotype
    IgG1
    Top Product
    Discover our top product KDELR Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml was sufficient for detection of KDEL receptor in 20 μg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    KDELR (KDEL Receptor (KDELR))
    Alternative Name
    KDEL Receptor (KDELR Products)
    Synonyms
    BEST:CK00230 antibody, CG5183 antibody, CK00230 antibody, Dmel\\CG5183 antibody, ERD2 antibody, Erd2 antibody, dmErd2 antibody, l(2)k00311 antibody, KDEL receptor antibody, KdelR antibody
    Background
    The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity, and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
    Gene ID
    618184
    NCBI Accession
    NP_001069963
    UniProt
    P33946
    Pathways
    Maintenance of Protein Location
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