p38 alpha MAPKinase antibody

Antigen: p38 alpha MAPKinase

Clonality: Monoclonal (9F12)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Catalog no.: ABIN361680

Additional Information

Characteristics: Detects a ~38kDa protein corresponding to the molecular mass of p38alpha MAPK on SDS PAGE immunoblots.

Gene ID: 1432

Swiss-Prot: Q16539

Immunogen: Full length recombinant protein expressed in E.coli cells.

Cross-Reactivity (Details): No known cross-reactivity with other related human kinases,

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9F12

Description: The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. This super family consists of stress activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases, each of which forms a separate pathway . The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2alpha translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death. Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP. p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs. As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis and has also been implicated in cancer, CNS and diabetes.
Synonyms: CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, MAPK14, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A

Specificity: Detects ~38kDa corresponding to the molecular mass of p38a MAPK on SDS-PAGE immunoblots. Species Cross-reactivity No known cross-reactivity with other related human kinases. Rat

Sensitivity: Detects ~38kDa protein corresponding to p38alpha MAPK when loaded with 6ng of purified p38alpha by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1:1000 (WB)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

p38 MAPkinase (9F12), human cell lines