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Details for Product No. ABIN361686

FK506 Binding Protein 4, 59kDa (FKBP4) antibody

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Antigen
Synonyms FKBP51, FKBP52, FKBP59, HBI, Hsp56, PPIase, p52, FKBP-4, FKBP-52, P59, 59kDa, AL022792, AW208983, FKPB52, p59, hsp56, fkbp52, fkbp59, MGC81078, wu:fb34f09
Reactivity
»Alternatives Dog (Canine), Hamster, Human, Rat (Rattus)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Pubmed 4 references available
Catalog no. ABIN361686
Quantity 25 µg
Price
201.30 $   Plus shipping costs $45.00
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Immunogen Synthetic peptide corresponding to the AA of human FKBP52
Clone Hi52C
Isotype IgG
Specificity Detects an approx. 52 kDa protein representing FKBP52 in cell lysate. Also detects FKBP52 in whole tissue extracts from rat kidney and rat and mouse testes. Heavy chain migrates close to FKBP52 on SDS PAGE.
Sensitivity 0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Purification Protein G Purified
Alternative Name FKBP52
Background Synonyms:
FK506 binding protein 4, FKBP4, FKBP59, HBI, Hsp56, p52, p59, PPIase, Rotamase, T cell FK506 binding protein
Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones. One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domainthat forms an Hsp90 binding site. Among the TPR co-chaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins. FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex. The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90.Although FKBP52 and FKBP51 share approx. 75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics. Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors.
Gene ID 2288
NCBI Accession NP_002005.1
UniProt Q02790
Research Area Chromatin and Nuclear Signaling, Cytoskeleton, Heat Shock Proteins
Application Notes Recommended Dilution: 1:2000 (WB), 5 µg with 10-20 µL Protein A beads (IP), 1:250 (IHC)
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Storage -20 °C
Background publications Davies, Ning, Sánchez: "A new first step in activation of steroid receptors: hormone-induced switching of FKBP51 and FKBP52 immunophilins." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4597-600, 2002 (PubMed).

Cheung-Flynn, Roberts, Riggs et al.: "C-terminal sequences outside the tetratricopeptide repeat domain of FKBP51 and FKBP52 cause differential binding to Hsp90." in: The Journal of biological chemistry, Vol. 278, Issue 19, pp. 17388-94, 2003 (PubMed).

Wu, Li, Liu et al.: "3D structure of human FK506-binding protein 52: implications for the assembly of the glucocorticoid receptor/Hsp90/immunophilin heterocomplex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 22, pp. 8348-53, 2004 (PubMed).

Denny, Prapapanich, Smith et al.: "Structure-function analysis of squirrel monkey FK506-binding protein 51, a potent inhibitor of glucocorticoid receptor activity." in: Endocrinology, Vol. 146, Issue 7, pp. 3194-201, 2005 (PubMed).

Alternatives for antigen "FK506 Binding Protein 4, 59kDa (FKBP4)", type "Antibodies"
Hosts (32), (31), (8)
Reactivities (68), (25), (23), (13), (13), (12), (7), (6), (4)
Applications (59), (29), (18), (16), (15), (10), (6), (5), (3), (3), (3), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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