Calcium/calmodulin-Dependent Protein Kinase II (CAMK2) antibody

Antigen: Calcium/calmodulin-Dependent Protein Kinase II (CAMK2)

Synonyms: CAMKA, KIAA0968, CaMKII, R74975, mKIAA0968, PKCCD, PK2CDD, CAMKII, CAMKIIalpha, CAMKIId, CDPK1, Ca2+/calmodulin-dependent protein kinase II, CaM, CaM II, CaM KII, CaMK II, CaMK-II, Calcium/calmodulin-dependent protein kinase, DCK, calcium/calmodulin-dependent protein kinase II, dCaMKII, DmelCG18069, CG18069, CAMK2A, MGC139375, MGC155201, MGC123320, zgc:112538, zgc:123320, Camkii, GB17817, DKFZp459L1326

Clonality: Monoclonal (22B1)

Host: Mouse

Reactivity: Rat (Rattus)

Application: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN361699

Additional Information

Alternative name: CaMKII

Immunogen: Synthetic peptide

Format: Protein G purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 22B1

Description: CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Specificity: Detects phosphorylated CaMKII from rat tissues. Monoclonal antibody 22B1 (anti-phospho-CaMKII is specific for a and

Synonyms: CAMKA, KIAA0968, CaMKII, R74975, mKIAA0968, PKCCD, PK2CDD, CAMKII, CAMKIIalpha, CAMKIId, CDPK1, Ca2+/calmodulin-dependent protein kinase II, CaM, CaM II, CaM KII, CaMK II, CaMK-II, Calcium/calmodulin-dependent protein kinase, DCK, calcium/calmodulin-dependent protein kinase II, dCaMKII, DmelCG18069, CG18069, CAMK2A, MGC139375, MGC155201, MGC123320, zgc:112538, zgc:123320, Camkii, GB17817, DKFZp459L1326

Application Details

Application Notes: 1u/ml of SMC-125 was sufficient for detection of 0.2ug CAMKII by colorimetric immunoblot

Concentration: 1.0mg/mL

Buffer: Mouse immunoglobulin in PBS pH 7.4, 0.09% sodium azide, in 50% glycerol. Protein G purified.

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: Cancer, Hypertrophy, Kinases/Phosphatases

Restrictions: For Research Use only

Images

CamKII (22B1), Mice ventricles

CamKII (22B1), dissociated hippocampal neurons

CamKII (22B1), Mouse backskin- muscle and hair follicle staining